Microscopes have been traditionally designed to use the human eye to observe the image. But nowadays more and more digital cameras are used as the imaging device in both standard and confocal microscopes. These new digital detectors are more sensitive to intensity and flatness differences than the human eye and require new strategies for objective lens design.
This article describes the optical performance of modern objective lenses and discusses criteria for the selection of objectives for a variety of microscope applications including fluorescence microscopy, confocal microscopy, and live cell imaging.
- Click on the "file attachment" link below to download the tutorial as a PDF.
