The new Gatan 3D tomography-acquisition software is a plug-in to the popular DigitalMicrograph software and works with Gatan’s complete line of room-temperature and cryotransfer tomography holders and Gatan Imaging Filters or digital TEM cameras on most TEMs (with computer controlled sample stages, illumination and imaging systems).
The Gatan tomography software allows you to acquire a tilt series while correcting for specimen drift and any mechanical instabilities of the goniometer. The tomography series can be collected with live tracking or using a pre-acquired stage calibration.The latter mode of operation minimizes the electron dose on the specimen and reduces acquisition time. After acquisition, the series can be played as a movie to highlight the 3D structure of the specimen or exported for reconstruction by any of the public domain packages available.
Some key acquisition features include: flexible setup for both novice and expert users; use of live track-Tomography Acquisition Plug-Ining of stage motion during data acquisition; use of aTilt-Induced Shift (TIS) algorithm to correct for changes in focus; tracking data can be used as a stage calibration in future acquisitions; pause acquisition of tilt series to adjust stage X, Y, or Z position, image shift, beam shift or defocus; manually assist the tracking algorithms when necessary; and image acquisition in TEM or EFTEM energy-filtered mode.You can also save and recall experiment setups and store image data in RAM and/or directly to disk.
