Volume 22, Issue 3 (May 2008)

Browse through the articles in Volume 22, Issue 3 (May 2008)...

Articles

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Spectrally Resolved Confocal Fluorescence Microscopy with a Supercontinuum Laser

S. Schlachter,1 A. Elder,1 J. H. Frank,2 A. Grudinin,3 C. F. Kaminski1
1. Dept. of Chemical Engineering, University of Cambridge, UK. 2. Combustion Research Facility, Sandia National Laboratories, Livermore, CA, USA. 3. Fianium Laser Systems, Southampton, UK
The advent of novel supercontinuum light sources is set to change the future of confocal
microscopy, enabling wavelength- and lifetime-resolved imaging. Here we describe
the development of a versatile confocal microscopy system which features full flexibility
for wavelength excitation and spectrally resolved fluorescence detection

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Use of Software to Enhance Depth of Field and Improve Focus in Photomicrography

Jörg Piper, Meduna-Klinik, Bad Bertrich, Germany
By using readily available free software or shareware, the focal depth and sharpness in
photomicrographic images can be enhanced dramatically. In this way, digital images containing three-dimensional information are achievable, comparable with scanning electron micrographs....

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Enhancement of Confocal Fluorescence Images of Neutrophils by Magnetic Fields

Elbert Reyes,1,2 Wilfredo Molina,1 Siham Salmen,3 Lisbeth Berrueta3 and Narahari Joshi1
1. Department of Physiology, 2. Department of Morphological Sciences, and 3. Institute of Clinical Immunology, University of Los Andes, Mérida, Venezuela

Fluorescent images of human neutrophil cells were obtained from confocal microscopy with and without the application of a magnetic field. This novel approach of imaging is found to be very useful for detecting cell structures, and also to reveal the morphological details which were not seen in images obtained without a magnetic field.

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Light, Confocal and Scanning Electron Microscopy of Wood-Adhesive Interface

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Adya Singh,1 Bernard Dawson,1 Catherine Rickard,1 Jacqueline Bond 2 and Anamika Singh1
1. Ensis, Rotorua, New Zealand. 2. Scion, Rotorua, New Zealand
A novel approach involving the combined use of light microscopy, confocal laser scanning microscopy and field-emission scanning electron microscopy was developed to examine the wood-adhesive interface in a
commercial plywood manufactured by gluing Pinus radiata wood plies with a phenolformaldehyde
adhesive...

Volume number: 
2008
Issue number: 
3

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