Volume 18, Issue 3 (May 2004)

We have devised a novel algorithm for quantitative immunohistochemistry (Q-IHC) that permits the absolute amount of chromogen present per pixel to be determined. Digital images are acquired; a region of interest is then selected, stored in TIFF format, and processed using our TIFFalyzer software...

A number of morbillivirus infection epidemics causing mass mortality have been reported in recent years in several free-living sea mammal species around the world. Their causative agents, at least three different morbilliviruses, were unknown in aquatic mammals before 1988...

Real-time machine vision studies of proliferation and apoptosis were performed over many days on living human cancer cells. 8x8 mozaics of monochrome VGA images in each well of 96-well plates provided proliferation and apoptosis results with appropriate statistical weight...

Specimens of the fresh water copepod Cyclops furcifer and the cladoceran Simocephalus exspinosus (Crustacea) have been used to compare two different modes of scanning electron microscopy: environmental scanning electron microscopy and scanning electron microscopy. The high level of moisture of these animals confers on them appropriate characteristics for such investigations, as they require drastic drying to be observed with conventional scanning microscopical methods...

This article highlights the importance of metallographic analysis in studying common casting defects, such as hot tears, cold cracks and localised stresses, on tin-bronzes. The microstructural features of two different compositions show the deep characterisation possibilities given by simple optical tint metallography...

Now that most transmission electron microscopes are computerised, there are a growing number of users who have amazingly little understanding of what actually goes on inside an electron column. For most purposes, doing what the computer and the instruction manual tell you to do is sufficient to get reasonable results...

Ion-mediated organic electronics is a prime example for the implementation of several microscopical techniques to study the phase composition and the morphological aspects of thin organic films consisting of mixed organic and inorganic components. In order to understand the device behaviour, it is crucial to be able to measure several parameters including surface roughness, the phase separation between the polymers, and the dissociation of the salt into ionic species as well as their distribution throughout the thin film...