Volume 16, Issue 5 (September 2002)

Extended depth of focus algorithms provide a set of very useful tools for obtaining in focus images of 3D objects and organisms. In this paper we review and compare three different classes of 2D extended depth of focus (EDF) algorithms: point, area and frequency domain based algorithms...

Colorectal carcinoma has a worldwide distribution, with the highest death rates in the United States and Eastern European countries. The aim of this study was to estimate sensitivity and specificity of karyometric method for early detection of colorectal carcinoma...

RIMAPS (Rotated Image with Maximum Average Power Spectrum) is a new surface characterization technique independent of the class of microscopy and conditions used for observation as long as they remain constant. This technique combines rotation of digitized images and Fourier transform to obtain the maximum value of any average power spectrum from each angular position...

Immunofluorescence methods for hormone-containing cells exposure are widely used but the quantitative nature of the immunostaining is frequently ignored. In this study, quantitative measurements of fluorescent pancreatic beta-cells before and after glucose stimulation were used to determine parameters characterizing insulin synthesis and secretory activity...

EBSD was used to optimise neutron strain measurement in friction stir (FS) welded aluminium alloy plates with a strong texture. Pole figures were generated for different regions in weld zones and parent material. For a given direction in the specimen, observed variation in population densities is in good agreement with the (111) peak intensity pattern obtained by neutron diffraction...

Since quantitative assays for estrogen (ER) and progesterone (PR) binding sites in the homogenate of human breast cancer biopsies are inaccurate due to the heterogeneity of cell population in small needle biopsies, cytochemistry of ER and PR is a method of choice to visualize small number of cancer cells positive for these binding sites. Our current study confirmed this hypothesis...