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From: Two-Photon Excitation for Optical Nanoscopy and Light-Sheet Illumination Microscopy Alberto Diaspro,1,2 Paolo Bianchini,1 Francesca Cella-Zanacchi,1 Benjamin Harke,1 Michela Perrone,1 Emiliano Ronzitti,1 Silvia Galiani,1,2 Jenu Chacko,1,2 and Zeno Lavagnino.1,2 1. IIT – Istituto Italiano di Tecnologia, Genoa, Italy. 2. LAMBS MicroSCoBio, Department of Physics, University of Genoa, Italy. Microscopy and Analysis 25(5):13-17 (AM), 2011

Figure 1e: Problems associated with conventional immunohistochemical labelling of whole mount hippocampal slice cultures. Slices cultured for 12 days were labelled with isolectin-b4 using conventional techniques and Z-series scans were taken using a confocal microscope. Slices were mounted using coverslip bridges to avoid squashing. Representative images of a whole mount slice are shown. Although labelling is clear near the top and bottom, there appears to be an unlabelled ‘blank space’ in the middle of the slice. The ‘blank space’ cannot be identified in serial coronal cryostat sections. The section shows the CA1 pyramidal cell payer. Red is MAP2, green connexin-43 and blue GFAP. Scale bar: 50 μm.

Figure 5a: One of a sequence of images showing the propagation of a crack in a polymer. The tensile axis is vertical. Radial cracks propagate within the spherulites. At the same time shear bands appear in the amorphous matrix. Once the crack reaches the spherulite amorphous interface, the fracture front follows along the shear bands. Scale bar = 500 μm. Source: Morphology of Plastic Deformation and Fracture of a Biodegradable Biopolymer. Élida Hermida1,2 and Vanesa Casariego1. 1. Institute of Technology Prof. Jorge A. Sabato, UNSAM-CNEA and Department of Materials, CNEA, Buenos Aires, Argentina. 2. CONICET, Buenos Aires, Argentina. Microscopy and Analysis 23(1):S5-S8 (AM), 2009.

Polarized light micrograph of ascorbic acid crystals

Guinea pig detrusor labelled with anti-PGP 9.5 and Alexa 488 (green) and anti-smooth muscle myosin and Alexa 594 (red).

Adhesive penetration into rays from the adhesive line (arrow) in Pinus radiata plywood.

Human U2OS osteosarcoma cell labelled with mCherry-Rad51 fluorescent protein.