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Recent Advances in Laser Technology for Laser Scanning Fluorescence Microscopy

Gail McConnell, Centre for Biophotonics, Strathclyde Institute for Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK

Microscopy and Analysis 21(5):19-23 (UK), 2007

ABSTRACT
As the life sciences communities continue to embrace confocal and multiphoton laser scanning fluorescence microscopy (CLSM and MPLSM), the growing range of applications places greater demands on laser technology. For example, with powerful imaging methods such as video-rate MPLSM and ratiometric CLSM becoming available, appropriate laser technology must be ready to fully capitalise upon these sophisticated techniques. Additionally, robust, simple to use and (ideally) inexpensive sources aresought to improve the efficiency and ease of existing imaging techniques. Nonlinear optics and new laser gain media and geometries may hold the key to creating useful solutions.

KEYWORDS
light microscopy, confocal microscopy, multiphoton microscopy, sources, lasers, nonlinear optics, photonic crystal fibres

Author:

Julian Heath

Publisher:

Microscopy and Analysis

Date:

13-Jul-08

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