March 2010 Europe Cover Picture and Story

The cover image is a section of human skin, stained for nuclei (DAPI, blue), keratin 1 (red) and keratin 14 (green), imaged with the Olympus FluoView FV1000 confocal laser scanning microscope. Image courtesy of Dr Frank Fischer and Dr Sonja Wessel, Beiersdorf AG, Hamburg, Germany.

With a multi-laser combiner providing up to six laser lines, the Olympus FluoView FV1000 cLSM can incorporating several modules for increased flexibility. In addition to providing highly resolved confocal images in up to six dimensions, the independent SIM scanner provides light stimulation while recording images with the main scanner. When fitted with the optional SIM scanner, the FV1000 can be used to measure the rapid kinetics of proteins in living cells. This SIM scanner uses the same laser lines as the main scanner, enabling simultaneous stimulation and imaging, with complete flexibility over wavelengths.

This advanced ‘optical bench’ approach also ensures that the FV1000 is perfect for simultaneous photobleaching studies such as FLIP and FRAP, as well as for photomanipulation studies, where fluorescent molecules can be activated or deactivated using defined wavelengths. Furthermore, regions of interest can be selected and stimulated during scanning to ensure accurate measurements are obtained during and immediately following stimulation.

The Olympus FluoView FV1000 can also be fitted with a TIRF module, which enables high resolution evanescent wave illumination of cell surface features. The advanced optics and flexibility of the FV1000 mean that the penetration depth of the evanescent wave can be carefully controlled to produce peerless TIRF images.

Contact:
OLYMPUS EUROPA HOLDING GMBH
Microscopy
Esther Ahrent
Department Manager Marketing Communications
Tel: +49 40 2 37 73 - 5426
Fax: +49 40 2 37 73 - 4647
E-mail: microscopy@olympus-europa.com
www.microscopy.olympus.eu