Two-photon microscopy is a hot topic in the poster sessions

The poster sessions included many two-photon microscopy topics.  Here’s a taste of some of the research presented:

*Researchers from Boston University and University Paris Descartes presented a technique that combines two-photon microscopy with two-photon activated caged Ca2.  Combining the real time calculation available from the dynamic current-clamp with precise two-photon uncaging let them induce an on-demand calcium release with dynamics matching those of an endogenous calcium-specific: the dynamic two-photon calcium-clamp.  Poster # 288.1/FF75.

*Researchers from the University of Michigan, Ann Arbor, and the University of Rochester combined in vivo two-photon imaging with extracellular multielectrode recording, which can potentially allow scientists to investigate questions related to the electrode-tissue interface. They used the method to examine the neocortex vasculature of the mouse in combination with extracellular recordings. Poster # 288.4/FF78

*Researchers from the Max-Planck-Institut fuer medizinische in Germany and Karolinska Institutet in Sweden used two-photon microscopy with bulk loading of AM-esters to measure population activity during sensory processing in layers 4 and 5 or a rodent barrel cortex. They were able to record deeper than accomplished before by using a regenerative amplifier. It provides high-energy pulses that compensate for the depth-induced reduction in focal light intensity. Poster#288.8/FF82