Olympus: Scale View System for Deep Multiphoton Imaging
At Neuroscience, Olympus America presented several new products, including: the Olympus Scaleview-A2 and 25x objective; the DP73 digital microscope camera; CellSens v.1.6 imaging software; high-speed microscope imaging solutions incorporating advanced cameras from Andor; and Olympus confocal / multiphoton microscope enhancements that provide faster scanning and focus, higher sensitivity, and the ability to acquire ultra-large images.
Brendan Brinkman, Product Manager for Laser Scanning Confocal Systems, talked to M&A Editor Dr Julian Heath about the new Scale View system. Scroll down to watch the video.
Scientists often study life processes by peering into biological organisms to see their structure and function. But brains and other tissues are almost all opaque, making it difficult to see deep inside. Now, thanks to a liquid that can literally make tissue transparent and minimizes light scatter, along with a new super-long-working-distance Olympus microscope objective, scientists are producing vivid 3D images of structures deep inside mouse brains and other animal organs. The reagent was developed by a research team at RIKEN Brain Science Institute in Japan; Olympus is now offering it, together with its specially designed 25x, NA1.0, 4mm-working-distance companion objective, to researchers in North and South America.
The SCALEVIEW-A2 reagent and SCALEVIEW 25x objective are designed to boost the capability of multiphoton microscopy and allow scientists to look deeper into tissue than they ever could before. In the mouse brain, for instance, the RIKEN team has imaged neurons and blood vessels down beyond the white matter, 4 mm beneath the surface. Until now, even advanced optical methods did not allow researchers to see this deep beneath the surface due to light scatter within the tissue. Furthermore, most optical techniques required slicing dead biological tissue into very thin sections, which damaged specimens and made it challenging to visualize exactly how slices fit together and, critically, how neural filaments connect throughout the brain.
http://www.olympusamerica.com/scaleview
Brendan Brinkman, Product Manager for Laser Scanning Confocal Systems, talks to M&A Editor Dr Julian Heath about the new Scale View system: